Genomics covers the three terms, transcriptomics, proteomics and metabolomics,
(or metabonomics) to describe the formation and fate of mRNA, proteins and metabolites,
respectively. Since organisms do not consist of isolated subsets of genes, proteins
and metabolites, the term systems biology has been coined. It refers to an integrated
approach to studying biological systems (e.g., intracellular networks, cells, organs, or
any biological entity) by measuring and integrating genetic, proteomic and metabolic data.
Together with our strategic partner, BG Medicine, Inc. (Waltham MA, USA), TNO leads
the field in these novel technologies. TNO offers a flexible and case-by-case project approach.
These range from fairly small-scale projects in transcriptomics and proteomics to integrated
projects in systems biology, which are carried out in cooperation with BG Medicine.
Biomarker Discovery
Genomics-based methods (transcriptomics, proteomics and metabolomics) combined with multivariate (pattern recognition) statistics form the basis for the discovery of novel biomarkers and biomarker patterns. Multivariate data analysis techniques such as Principal Component Analysis (PCA) and PCA coupled with discriminant analysis (PCDA) are very powerful tools for analysing data and detecting clusters of similar datasets as well as differences between datasets. We have a long-standing tradition in assay development and the analysis of biomolecules, and our laboratories enable us to combine various biochemical and molecular platforms with state-of-the-art analytical instrumentation
Tailor-made solutions in proteomics
is Proteomics is group of technologies that are used to identify and compare proteins as well as to determine posttranslational modifications, sub-cellular location and molecular interactions. We apply technologies that are suited to a specific question, offering a comprehensive set of proteomics technologies which can be used to answer specific questions. We will try to find the needle in the haystack, not by looking randomly through the entire haystack to find the needle, but by focusing on those parts where it is most likely to be located. Although 2D electrophoresis is an arrived technique in combination with the newest fluorescent labeling techniques (2D DIGE) it enables us to accurately quantify even the smallest amount of protein. Using these in combination with LT-Q-FTMS and Nano-electrospray MS, we can help you to identify the most relevant proteins.
Reference publications
The Role of Analytical Sciences in Medical Systems Biology Current Opinion
in Chemical
Biology 2004
(or metabonomics) to describe the formation and fate of mRNA, proteins and metabolites,
respectively. Since organisms do not consist of isolated subsets of genes, proteins
and metabolites, the term systems biology has been coined. It refers to an integrated
approach to studying biological systems (e.g., intracellular networks, cells, organs, or
any biological entity) by measuring and integrating genetic, proteomic and metabolic data.
Together with our strategic partner, BG Medicine, Inc. (Waltham MA, USA), TNO leads
the field in these novel technologies. TNO offers a flexible and case-by-case project approach.
These range from fairly small-scale projects in transcriptomics and proteomics to integrated
projects in systems biology, which are carried out in cooperation with BG Medicine.
Biomarker Discovery
Genomics-based methods (transcriptomics, proteomics and metabolomics) combined with multivariate (pattern recognition) statistics form the basis for the discovery of novel biomarkers and biomarker patterns. Multivariate data analysis techniques such as Principal Component Analysis (PCA) and PCA coupled with discriminant analysis (PCDA) are very powerful tools for analysing data and detecting clusters of similar datasets as well as differences between datasets. We have a long-standing tradition in assay development and the analysis of biomolecules, and our laboratories enable us to combine various biochemical and molecular platforms with state-of-the-art analytical instrumentation
Tailor-made solutions in proteomics
is Proteomics is group of technologies that are used to identify and compare proteins as well as to determine posttranslational modifications, sub-cellular location and molecular interactions. We apply technologies that are suited to a specific question, offering a comprehensive set of proteomics technologies which can be used to answer specific questions. We will try to find the needle in the haystack, not by looking randomly through the entire haystack to find the needle, but by focusing on those parts where it is most likely to be located. Although 2D electrophoresis is an arrived technique in combination with the newest fluorescent labeling techniques (2D DIGE) it enables us to accurately quantify even the smallest amount of protein. Using these in combination with LT-Q-FTMS and Nano-electrospray MS, we can help you to identify the most relevant proteins.
Reference publications
The Role of Analytical Sciences in Medical Systems Biology Current Opinion
in Chemical Biology 2004
